SubtiBank SubtiBank
cwlS
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

cwlS

D,L-endopeptidase, peptidoglycan hydrolase for cell separation
Locus
BSU_19410
Isoelectric point
10.44
Molecular weight
44.07 kDa
Protein length
414 aa Sequence Blast
Gene length
1245 bp Sequence Blast
Function
cell separation
Product
D,L-endopeptidase, peptidoglycan hydrolase
Essential
no
Synonyms
yojL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
Loading

Categories containing this gene/protein

  • Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → Autolysis
  • Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → Endopeptidases

    • Gene

    Coordinates
    2,115,425 2,116,669

    The protein

    Catalyzed reaction/ biological activity

  • cleaves the peptide bond between D-Glu (position 2 in the peptioglycan peptide) and m-diamino pimelic acid (position 3) PubMed
  • degradation of gamma-polyglutamic acid PubMed
  • Hydrolysis of gamma-D-glutamyl bonds to the L-terminus (position 7) of meso-diaminopimelic acid (meso-A(2)pm) in 7-(L-Ala-gamma-D-Glu)-meso-A(2)pm and 7-(L-Ala-gamma-D-Glu)-7-(D-Ala)-meso-A(2)pm. It is required that the D-terminal amino and carboxy groups of meso-A(2)pm are unsubstituted (according to UniProt)

    • Protein family

  • Peptidase C40 family (according to UniProt)

    • Domains

  • contains four N-acetylglucosamine-polymer-binding LysM domains PubMed
  • C-terminal D,L-endopeptidase domain (NlpC/P60 domain) PubMed
  • 4 LysM domains (aa 27-70, aa 88-131, aa 157-200, aa 225-268) (according to UniProt)

    • Effectors of protein activity

  • both enzymatic activities are inhibited by interaction with IseA PubMed

    • Structure

  • 4XCM (from Thermus thermophilus, 34% identity) PubMed

    • Localization

  • localizes to cell septa and poles PubMed

    • Expression and Regulation

    Operons

    Genes
    cwlS
    Description
    PubMed

    Sigma factors

  • SigD: sigma factor, PubMed, in SigD regulon
  • SigH: sigma factor, PubMed, in SigH regulon

    • Regulatory mechanism

  • CcpA: repression, [pubmed|], in CcpA regulon
  • Abh: repression, synergistic transcription repression with AbrB PubMed, in Abh regulon
  • AbrB: repression, synergistic transcription repression with Abh PubMed, in AbrB regulon

    • Regulation

  • repressed by glucose (3-fold) (CcpA) PubMed
    • view in new tab

    Genes
    cwlS-yojM
    Description
    PubMed
    view in new tab

    Biological materials

    Mutant

  • MGNA-B424 (yojL::erm), available at the NBRP B. subtilis, Japan
  • BKE19410 (cwlS::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCATTCAACCTCCTAAA, downstream forward: _UP4_TAAAAATGAGTATGACAAAA
  • BKK19410 (cwlS::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCATTCAACCTCCTAAA, downstream forward: _UP4_TAAAAATGAGTATGACAAAA

    • References

    Reviews

    Loading

    Original publications

    Loading